rabbit anti socs3  (Cell Signaling Technology Inc)

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: PCV2 infection upregulated the mRNA and protein levels of SOCS3 in PK-15 cells. ( a ) PK-15 cells were inoculated with PCV2 at a MOI of 1.0 or mock-infected for 0, 12, 24, and 48 h and analyzed by qRT-PCR measurements of the SOCS3 mRNA. ( b ) Immunoblotting with anti-SOCS3 and anti-β-actin antibodies to measure the SOCS3 protein at 0, 12, 24, and 48 hpi, respectively. Data are presented as mean ± SD ( n = 3). * p < 0.05, *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Infection, Quantitative RT-PCR, Western Blot, Control

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: SOCS3 expression facilitates PCV2 replication. ( a ) Fluorescence imaging of PK-15 cells transfected with varying concentrations of Cy3-labeled siRNA. ( b ) SOCS3 mRNA expression levels and ( c ) corresponding knockdown efficiency at different time points post-siRNA transfection. ( d ) Fluorescence visualization of HEK-293T cells at distinct time intervals following lentiviral vector transduction. ( e ) Fluorescence patterns of SOCS3-stably transfected cells and blank controls after 10 consecutive passages. ( f ) Relative SOCS3 mRNA expression levels in stably transfected cells. ( g ) Cell viability analysis of SOCS3-silenced and overexpressing cell lines. ( h ) Protein expression profiles in SOCS3-knockdown and overexpression models. ( i ) Absolute quantitative PCR analysis of PCV2 viral copy numbers in wild-type PK-15, si-NC, and si-SOCS3-2 cells infected with PCV2 (MOI = 1) at indicated time points. ( j ) PCV2 viral load quantification in wild-type PK-15, PK-15-pCDH, and PK-15-SOCS3 cells post-infection. ( k ) Indirect immunofluorescence detection of PCV2 infection status across experimental groups. Data are presented as mean ± SD ( n = 3). *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Expressing, Fluorescence, Imaging, Transfection, Labeling, Knockdown, Plasmid Preparation, Transduction, Stable Transfection, Over Expression, Real-time Polymerase Chain Reaction, Infection, Immunofluorescence, Control

Journal: Viruses

Article Title: PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells

doi: 10.3390/v17081081

Figure Lengend Snippet: Effects of SOCS3 on PCV2-induced IFN-β and TNF-α mRNA expressions. ( a ) SOCS3 silencing suppresses IFN-β production in PCV2-infected host cells while ( b ) enhancing TNF-α expression. Relative quantitative PCR analysis of IFN-β and TNF-α mRNA levels in wild-type PK-15, si-NC, and si-SOCS3-2 cells infected with PCV2 (MOI = 1) at indicated time points. ( c ) SOCS3 overexpression promotes IFN-β production in PCV2-infected host cells, whereas ( d ) it inhibits TNF-α expression. Relative quantitative PCR analysis of IFN-β and TNF-α mRNA levels in wild-type PK-15, PK-15-pCDH, and PK-15-SOCS3 cells post-PCV2 infection (MOI = 1). Data are presented as mean ± SD ( n = 3). ** p < 0.01, *** p < 0.001 vs. control.

Article Snippet: Immunoblotting was performed using a rabbit polyclonal anti-SOCS3 antibody (Proteintech; 1:1000 dilution) incubated overnight at 4 °C, followed by incubation with a HRP-conjugated goat anti-rabbit secondary antibody (Servicebio; 1:3000 dilution) for 1 h at room temperature.

Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Over Expression, Control

Journal: Frontiers in Immunology

Article Title: Expression and correlation of SOCS3 and Eotaxin mRNA and proteins levels in nasal mucosal tissue of allergic rhinitis patients

doi: 10.3389/fimmu.2025.1561650

Figure Lengend Snippet: Expression of SOCS3 and Eotaxin proteins in the AR group and the control group detected by immunohistochemical method.

Article Snippet: The rabbit anti-human SOCS3 antibody (catalog No. A01234, GenScript, Nanjing, China) was purchased from GenScript Company, located in Nanjing, China, and the rabbit anti-human Eotaxin protein antibody (catalog No. sc-56890, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased from Santa Cruz Company in Dallas, TX, USA.

Techniques: Expressing, Control, Immunohistochemical staining

Journal: Frontiers in Immunology

Article Title: Expression and correlation of SOCS3 and Eotaxin mRNA and proteins levels in nasal mucosal tissue of allergic rhinitis patients

doi: 10.3389/fimmu.2025.1561650

Figure Lengend Snippet: Correlation analysis of the expression levels of SOCS3 and Eotaxin proteins in the AR group.

Article Snippet: The rabbit anti-human SOCS3 antibody (catalog No. A01234, GenScript, Nanjing, China) was purchased from GenScript Company, located in Nanjing, China, and the rabbit anti-human Eotaxin protein antibody (catalog No. sc-56890, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased from Santa Cruz Company in Dallas, TX, USA.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Expression and correlation of SOCS3 and Eotaxin mRNA and proteins levels in nasal mucosal tissue of allergic rhinitis patients

doi: 10.3389/fimmu.2025.1561650

Figure Lengend Snippet: The expression of SOCS3 mRNA and Eotaxin mRNA in nasal mucosal tissues of the two groups of patients was detected by RT-PCR (A) Expression level of Eotaxin mRNA, (B) Expression level of SOCS3 mRNA.

Article Snippet: The rabbit anti-human SOCS3 antibody (catalog No. A01234, GenScript, Nanjing, China) was purchased from GenScript Company, located in Nanjing, China, and the rabbit anti-human Eotaxin protein antibody (catalog No. sc-56890, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased from Santa Cruz Company in Dallas, TX, USA.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: Expression and correlation of SOCS3 and Eotaxin mRNA and proteins levels in nasal mucosal tissue of allergic rhinitis patients

doi: 10.3389/fimmu.2025.1561650

Figure Lengend Snippet: Correlation analysis of SOCS3 mRNA and Eotaxin mRNA in nasal mucosal tissues of AR patients.

Article Snippet: The rabbit anti-human SOCS3 antibody (catalog No. A01234, GenScript, Nanjing, China) was purchased from GenScript Company, located in Nanjing, China, and the rabbit anti-human Eotaxin protein antibody (catalog No. sc-56890, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased from Santa Cruz Company in Dallas, TX, USA.

Techniques: